SmartFlares

Project to investigate the mechanism of action of SmartFlare RNA detection probes. Data linked to the open notebook at: [https://raphazlabcommons.wordpress.com] and licensed under the Creative Commons Attribution 4.0 International License [http://creativecommons.org/licenses/by/4.0/].

• 2015-02-26-NanoFlareLoadingCtrl Load All Images
  
  First experiment looking at the Uptake Control (Cy5 labelled) in HeLa cells at the 18h timepoint.
  
• 2015-03-03-TEM-UptakeControl Load All Images
  
  Transmission Electon Micrographs of the Uptake Control SmartFlares, acquired by Joan Comenge.
  
• 2015-03-10-NanoFlareLive Load All Images
  
  
• 2015-03-18-NanoFlareFixed.mdb Load All Images
  
  First fixed experiment looking at co-localising SmartFlares with various organelles.
  
• 2015-03-19-NanoFlareLive.mdb Load All Images
  
  
• 2015-04-15-NanoFlaresLive.mdb Load All Images
  
  
• 2015-04-22-NanoFlaresLive_2h.mdb Load All Images
  
  Cells imaged at identical acquisition settings to 18h data set and also at a best contrast setting (hi channels)
  
• 2015-04-22-NanoFlaresLive.mdb Load All Images
  
  
• 2015-05-05-SF_TEM Load All Images
  
  Transmission Electron Microscopy of VEGF and Scrambled SmartFlares. Data Courtesy of Joan Comenge
  
• 2015-05-22-NanoFlaresLive_Chloroquin.mdb Load All Images
  
  Experiment to see how the addition of (100uM) Chloroquine alters the localisation and/or intensity of VEGF SmartFlares.
  
• 2015-06-03-VEGFNanoFlaresFixed.mdb Load All Images
  
  Experiment to explore the localisation of VEGF SmartFlares through immunostaining and co-localisation analysis.
  
• 2015-06-04-NanoFlaresLive_Chloro_HiCont.mdb Load All Images
  
  
• 2015-06-04-NanoFlaresLive_Chloro.mdb Load All Images
  
  A repeat of the chloroquine experiment +/- DMOG to see if the Chloroquine is disrupting endosomes. Blog post at https://raphazlabcommons.wordpress.com/2015/06/08/effect-of-chloroquine-on-smartflare-distribution-and-intensity/ Interpretation of the data at https://raphazlabcommons.wordpress.com/2015/06/10/interpretation-of-chloroquine-results/
  
• 2015-06-19-Dextran_Chloroquine.mdb Load All Images
  
  Experiment to probe the source of the Chloroquine-mediated swollen vesicles. HeLa Cells were loaded for 24h with Red Dextran followed by a wash and 24h with Green Dextran +/- Chloroquine
  
• 2015-06-26-microinjection Load All Images
  
  Direct injection of SmartFlares into the cytosol. SFs made up 1:1 with 10mg/mL dextran. Injected using a Femtotip 2 (eppendorf) with a compensation pressure of 75hPa.
  
• 2015-07-04-VEGF_NanoFlares_TEM Load All Images
  
  Imaging the localisation of VEGF SmartFlares inside cells using TEM. Data courtesy of Joan Comenge.
  
• 2015-07-17-UC_NanoFlares_TEM Load All Images
  
  TEM data for Uptake Control SmartFlares. Cells were exposed to SmartFlares for 18h then prepared for EM.
  
• 2015-07-30-NanoFlaresFixed_Cav1.mdb Load All Images
  
  Testing the Santa Cruz anti-Cav1 antibody in HeLa cells loaded with FITC dextran.
  
• 2015-09-23-StickyFlaresDataRequest Load All Images
  
  Data were requested from Chad Mirkin for Figure 2 and Figure 4/ Supp Movie 1 from the StickyFlare paper (doi:10.1073/pnas.1510581112)
  
• 2015-12-01-JOVE-Lahm Load All Images
  
  Composite figure extracted from a JOVE article
  
• 2016-05-16-IJO-AmatyaDataRequest Load All Images
  
  Data request for figure 2 images. DOI: 10.3892/ijo.2016.3358